Rudolf Jaenisch, MD

Member, Whitehead Institute Professor of Biology, MIT 617.258.5186 phone 617.258.6505 fax kemske@wi.mit.edu

June 6 — Jaenisch lab has manipulated mouse fibroblasts and turned them into cells with such developmental elasticity that they appear identical to embryonic stem cells. Read more >

Rudolf Jaenisch is a Founding Member of Whitehead Institute and a pioneer of transgenic science, in which researchers alter an animal’s genetic makeup to produce a variant of a human disease. Jaenisch has focused on creating transgenic mice that enable his lab to study forms of cancer and neurological diseases that have long baffled researchers.

Jaenisch’s first breakthrough occurred in the 1970s when he demonstrated for the first time that foreign DNA could be integrated into the DNA of early mouse embryos; mice derived from these embryos carried the foreign genes in all of their tissues. Subsequently, Jaenisch injected retrovirus into early mouse embryos and showed that leukemia DNA sequences had integrated not only into the mouse genome but also to its offspring. These mice were the first transgenic animals in history.

Jaenisch also is a leader in the field of therapeutic cloning, also called nuclear transfer, in which the genetic information from one cell is transplanted into an unfertilized egg from which DNA has been removed. When placed in a Petri dish, this egg develops into a blastocyst from which stem cells can be taken. Jaenisch’s therapeutic cloning research occurs exclusively with mice, but he advocates using the same techniques with human cells so that we may one day develop therapies for diseases that are currently untreatable. [ therapeutic cloning 220 kbps QuickTime] Conversely, Jaenisch opposes human reproductive cloning, in which the egg is placed not in a Petri dish but into the uterus of a female, in the hope that it will develop into a fetus. [ reproductive cloning 220 kbps QuickTime] Recently, using mice as a model, Jaenisch demonstrated that it is possible to procure embryonic stem cells without harming a viable embryo. Using a technique called "altered nuclear transfer," Jaenisch created an embryo-like entity that was genetically incapable of implanting in a uterus, and that also had certain structural deficiencies. Although this entity was not a viable embryo, it did yield perfectly healthy embryonic stem cells. If this research is successfully repeated in human cells, it might provide a solution to the ethical debate.

Today, the over-arching goal of Jaenisch’s lab is to understand what scientists call epigenetic regulation of gene expression. This refers to the biological mechanisms that affect how genetic information is converted into cell structures but that don’t alter the genes in the process. Jaenisch’s lab is also investigating epigenetic mechanisms for certain types of cancer and for brain development, where much of his work focuses on how conditions such as Rett Syndrome occur.

Jaenisch received his doctorate in medicine from the University of Munich in 1967. Before coming to Whitehead, he was head of the Department of Tumor Virology at the Heinrich Pette Institute at the University of Hamburg. He has coauthored more than 300 research papers and has received numerous prizes and recognitions including an appointment to the National Academy of Sciences in 2003.

Selected Publications

Meissner, A., Jaenisch, R. (2005) Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts. Nature, October 16, 2005, early online edition.

Eggan K, Baldwin K, Tackett M, Osborne J, Gogos J, Chess A, Axel R, Jaenisch R. (2004). Mice cloned from olfactory sensory neurons. Nature. 428(6978):44-9.

Hochedlinger, K. & Jaenisch, R. (2002). Generation of monoclonal mice by nuclear transfer from mature B and T donor cells. Nature 415, 1035-1038.

Rideout, W.M., Hochedlinger, K., Kyba, M., Daley, G., & Jaenisch, R. (2002). Correction of a genetic defect by nuclear transfer and combined cell and gene therapy. Cell 109, 17-27.

Schnieke, A., Harbers, K., Jaenisch, R. (1983). Embryonic lethal mutation in mice induced by retrovirus insertion into the _1(I) collagen gene. Nature 304, 315-320.

Jaenisch, R., Jähner, D., Nobis, P., Simon, I., Löhler, J., Harbers, K., Grotkopp, D. (1981). Chromosomal position and activation of retroviral genomes inserted into the germ line of mice. Cell 24, 519-529.

Jaenisch, R. (1976). Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc. Natl. Acad. Sci. USA 73, 1260-1264.

Jaenisch, R., Mintz, B. (1974). Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc. Natl. Acad. Sci. USA 71, 1250-1254.

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